Amino Acid Analysis by Precolumn Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-L-Alanine Amide (Marfey’s Reagent)

  • Sunil Kochhar
  • Barbara Mouratou
  • Philipp Christen
Part of the Springer Protocols Handbooks book series (SPH)


Precolumn modification of amino acids and the subsequent resolution of their derivatives by reverse-phase high-performance liquid chromatography (RP-HPLC) is now the preferred method for quantitative amino acid analysis. The derivatization step introduces covalently bound chromophores necessary not only for interactions with the apolar stationary phase for high resolution but also for photometric or fluorometric detection.


Cysteic Acid Precolumn Derivatization Enantiomeric Amino Acid Quantitative Amino Acid Analysis Amino Acid Standard Solution 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Sunil Kochhar
    • 1
  • Barbara Mouratou
    • 2
  • Philipp Christen
    • 2
  1. 1.Nestlé Research CenterLausanneSwitzerland
  2. 2.Biochemisches Institut der Universität ZürichSwitzerland

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