Chemical Cleavage of Proteins at Tryptophanyl-X Peptide Bonds
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Tryptophan is represented in the genetic code by a single codon and has proven useful in cloning exercises in providing an unambiguous oligonucleotide sequence as part of a probe or primer. It is also one of the less abundant amino acids found in polypeptides, and cleavage of bonds involving tryptophan generates large peptides. This may be convenient for generation of internal sequence information (the usual purpose to which this technique is put), but is less useful for identification of proteins by mass mapping, where a larger number of smaller peptides makes for more successful database searching.
KeywordsFume Hood Leader Peptide Cyanogen Bromide Usual Purpose Large Peptide
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