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Modification of Arginine Side Chains with p-Hydroxyphenylglyoxal

  • Dan S. Tawfik
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

A variety of dicarbonyl compounds including phenylglyoxal, 2,3-butanendione, and 1,2-cyclohexanedione selectively modify the guanidine group of arginine (1-3). The main advantage of p-hydroxyphenylglyoxal is in the ability to determine the number of modified arginines spectrophotometrically. This reagent is also reactive at mildly alkaline pH (usually 8.0-9.0) and yields a single product that is relatively stable (1).

Keywords

Deionized Water Protein Solution Maximal Absorbance Single Product Molar Excess 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Yamasaki, R. B., Vega, A., and Feeney, R. E. (1980) Modification of available arginine residues in proteins by p-hydroxyphenylglyoxal. Analyt. Biochem. 109, 32–40.PubMedCrossRefGoogle Scholar
  2. 2.
    Rogers, T. B., Børresen, T., and Feeney, R. E. (1978) Chemical modification of the arginines in transferrins. Biochemistry 17, 1105–1109.PubMedCrossRefGoogle Scholar
  3. 3.
    Stoughton, D. M., Zapata, G., Picone, R., and Vann, W. F. (1999) Identification of Arg-12 in the active site of Escherichia coli K1CMP-sialic acid synthetase. Biochem. J. 343, 397–402.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Dan S. Tawfik
    • 1
  1. 1.Department of Biological Chemistrythe Weizman Institute of ScienceRehovotIsrael

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