Modification of Arginine Side Chains with p-Hydroxyphenylglyoxal
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A variety of dicarbonyl compounds including phenylglyoxal, 2,3-butanendione, and 1,2-cyclohexanedione selectively modify the guanidine group of arginine (1-3). The main advantage of p-hydroxyphenylglyoxal is in the ability to determine the number of modified arginines spectrophotometrically. This reagent is also reactive at mildly alkaline pH (usually 8.0-9.0) and yields a single product that is relatively stable (1).
KeywordsDeionized Water Protein Solution Maximal Absorbance Single Product Molar Excess