Side Chain Selective Chemical Modifications of Proteins

  • Dan S. Tawfik
Part of the Springer Protocols Handbooks book series (SPH)


Chemical modifications of proteins may be performed simply and rapidly to provide preliminary data regarding the role of particular amino acids in a given protein. Many reviews and books cover these aspects of protein chemistry; only a few are cited here (1-4). In particular, the book by Means and Feeney (1), although about 30 yr old, is an excellent introduction and a practical guide to this field. Notably, even in the era of molecular biology, when site-directed mutagenesis has become widely accessible (including with non-natural amino acids), selective chemical modifications are still applied regularly. In most cases, chemical modifications are used, often together with site-directed mutagenesis, to either identify or confirm the role of active site residues (for recent examples see ref. 5-9). But chemical modifications are also applied for the generation of improved and modified proteins for a variety of applications (10-12).


Chemical Modification Active Site Residue Amino Acid Side Chain Modification Reaction Diethyl Pyrocarbonate 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Dan S. Tawfik
    • 1
  1. 1.Department of Biological Chemistrythe Weizman Institute of ScienceRehovotIsrael

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