Pyridylethylation of Cysteine Residues

  • Malcolm Ward
Part of the Springer Protocols Handbooks book series (SPH)


To help maintain their three-dimensional structure, many proteins contain disulfide bridges between cysteine residues. Cysteine residues can cause problems during Edman sequence analysis, and quantification of cysteine and cystine by amino acid analysis is difficult since these residues are unstable during acid hydrolysis.


Cysteine Residue Amino Acid Analysis Disulfide Bridge Laser Desorption Ionization Guanidine Hydrochloride 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Malcolm Ward
    • 1
  1. 1.Proteome Sciences plcKings CollegeLondonUK

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