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Performic Acid Oxidation

  • Alastair Aitken
  • Michèle Learmonth
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Where it is necessary to carry out quantitative amino acid analysis of cysteine or cystine residues, carboxymethylation ( Chapter 59) or pyridethylation ( Chapter 62) is not the method of choice as it may be difficult to assess the completeness of the reactions. It is often preferable to oxidize the thiol or disulfide groups with performic acid to give cysteic acid. This also has the effect of converting methionine residues to methionine sulfone. Tyrosine residues may be protected from reaction by the use of phenol in the reaction mixture.

Keywords

Tyrosine Residue Derivatized Amino Acid Disulfide Linkage Cysteic Acid Final Protein Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Alastair Aitken
    • 1
  • Michèle Learmonth
    • 2
  1. 1.Division of Biomedical and Clinical Laboratory Sciences, Membrane Biology GroupUniversity of EdinburghScotland, UK
  2. 2.Department of Biomedical SciencesUniversity of EdinburghScotland

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