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Preparation of Avidin Conjugates

  • Rosaria P. Haugland
  • Mahesh K. Bhalgat
Protocol
  • 152 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The high-affinity avidin-biotin system has found applications in different fields of biotechnology including immunoassays, histochemistry, affinity chromatography, and drug delivery, to name a few. A brief description of avidin and avidin-like molecules, streptavidin, deglycosylated avidin, and NeutrAvidin™ avidin, is presented in  Chapter 48. With four biotin binding sites per molecule, the avidin family of proteins is capable of forming tight complexes with one or more biotinylated compounds (1). Typically, the avidin-biotin system is used to prepare signal-amplifying ”sandwich“ complexes between specificity reagents (e.g., antibodies) and detection reagents (e.g., fluorophores, enzymes, and so on). The specificity and detection reagents are independently conjugated, one with avidin and the other with biotin, or both with biotin, providing synthetic flexibility (2).

Keywords

Succinic Anhydride Biotin Binding Thioether Bond Affinity Matrice Biotin Binding Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Rosaria P. Haugland
    • 1
  • Mahesh K. Bhalgat
    • 2
  1. 1.Molecular Probes Inc.Eugene
  2. 2.Molecular Probes, Inc.Eugene

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