Alkaline Phosphatase Labeling of IgG Antibody
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Springer Protocols Handbooks
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Alkaline phosphatase (EC 188.8.131.52) from bovine intestinal mucosa is a valuable label. It is stable, has a moderate size (140 kDa), a high turnover number, and can be assayed using a variety of different substrates which are measured by changes in absorbance, fluorescence, or luminescence. The most common method of labeling immunoglobulin G (IgG) antibody with this enzyme uses the homobifunctional reagent glutaraldehyde.
KeywordsAlkaline Phosphatase Free Thiol Group Short Glass Maleimide Group Transfer Loss
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