Autoradiography and Fluorography of Acrylamide Gels

  • Antonella Circolo
  • Sunita Gulati
Part of the Springer Protocols Handbooks book series (SPH)


Autoradiography detects the distribution of radioactivity on gels or filters by producing permanent images on photographic film. It is frequently used in a variety of experimental techniques ranging from Southern and Northern blot analysis (1), to visualization of radioactive proteins separated in a sodium dodecyl sulfate (SDS)-Poly-acrylamide gels (2), to detection of nuclear factors bound to a labeled DNA probe in gel shift analysis (3), and to localization of DNA bands in sequencing gels (4).


Whatman Paper Acetic Acid Glacial Plastic Wrap Cupric Sulfate Radioactive Band 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Antonella Circolo
    • 1
  • Sunita Gulati
    • 1
  1. 1.Maxwell Finland Lab for Infectious DiseasesBoston

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