Detection of Proteins in Polyacrylamide Gels by Fluorescent Staining

  • Michael J. Dunn
Part of the Springer Protocols Handbooks book series (SPH)


Techniques of polyacrylamide gel electrophoresis are often the method of choice for the analysis of patterns of gene expression in a wide variety of complex systems. In particular, two-dimensional polyacrylamide gel electrophoresis (2-DE) is the core technology for separating complex protein mixtures in the majority of proteome projects (1). This is due to its unrivalled power to separate simultaneously thousands of proteins and the availability of sophisticated computer software for the qualitative and quantitative analysis of differential patterns of protein expression (2). Once this analysis has indicated proteins of interest, it is now relatively straightforward to directly identify and characterize proteins from polyacrylamide gels using highly sensitive microchemical methods (3), particularly those based on mass spectrometry (4). This has resulted in gel electrophoresis becoming one of the most important methods of protein purification for subsequent identification and characterization.


Silver Staining High Dynamic Range Dansyl Chloride Sophisticated Computer Software Complex Protein Mixture 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Michael J. Dunn
    • 1
  1. 1.Department of NeuroscienceInstitute of PsychiatryLondonUK

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