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Detection of Serological Cross-Reactions by Western Cross-Blotting

  • Peter Hammerl
  • Arnulf Hartl
  • Johannes Freund
  • Josef Thalhamer
Protocol
  • 73 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Antisera are frequently used tools for the characterization of proteins and peptides because they provide unique information about the structural features of antigens. Proteins that share structural similarities can be identified by serological cross-reactions. The Western blotting technique combines two steps that are characteristic for immunoaffinity chromatography (1). One is the preparation of monospecific antibodies against a particular antigen and the second is the testing of their reactivity with the same or different antigens from any source of antigenic material.

Keywords

Dialysis Membrane Western Blotting Technique Immunoaffinity Chromatography Monospecific Antibody Laboratory Shaker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Hammerl, P., Hartl, A., and Thalhamer, J. (1992) A method for the detection of serologically crossreacting antigens both within and between protein mixtures: the Western cross blot. J. Immunol. Meth. 151, 299–306.CrossRefGoogle Scholar
  2. 2.
    Beall, J. A. and Mitchell, J. F. (1986) Identification of a particular antigen from a parasite cDNA library using antibodies affinity purified from selected portions of Western blots. J. Immunol. Meth. 86, 217–223.CrossRefGoogle Scholar
  3. 3.
    Laemmli, E. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Peter Hammerl
    • 1
  • Arnulf Hartl
    • 1
  • Johannes Freund
    • 1
  • Josef Thalhamer
    • 1
  1. 1.Institute of Chemistry and Biochemistry, Immunology GroupUniversity of SalzburgAustria

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