Purification of IgG Using Thiophilic Chromatography
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Immunoglobulins recognize sulphone groups in close proximity to a thioether group (1), and, therefore, thiophilic adsorbents provide an additional chromatographic method for the purification of immunoglobulins that can be carried out under mild conditions preserving biological activity. A thiophilic gel is prepared by reducing divinylsulfone (coupled to Sepharose 4B) with β-mercaptoethanol. The product is of intermediate purity and would be useful for further processing, e.g., purification by ion-exchange, size exclusion, and/or affinity chromatography.