Purification of IgG Using Thiophilic Chromatography

  • Mark Page
  • Robin Thorpe
Part of the Springer Protocols Handbooks book series (SPH)


Immunoglobulins recognize sulphone groups in close proximity to a thioether group (1), and, therefore, thiophilic adsorbents provide an additional chromatographic method for the purification of immunoglobulins that can be carried out under mild conditions preserving biological activity. A thiophilic gel is prepared by reducing divinylsulfone (coupled to Sepharose 4B) with β-mercaptoethanol. The product is of intermediate purity and would be useful for further processing, e.g., purification by ion-exchange, size exclusion, and/or affinity chromatography.


Sodium Azide Binding Buffer Sodium Carbonate Ascitic Fluid Vacuum Filtration 
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  1. 1.
    Porath, J., Maisano, F., and Belew, M. (1985) Thiophilic adsorption-a new method for protein fractionation. FEBS Lett. 185, 306–310.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Mark Page
    • 1
  • Robin Thorpe
    • 2
  1. 1.Apovia Inc.San Diego
  2. 2.Division of ImmunobiologyNational Institute for Biological Standards and ControlPotters BarUK

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