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Isolation of Mouse Spleen Dendritic Cells

  • Andrew J. Stagg
  • Fiona Burke
  • Suzanne Hill
  • Stella C. Knight
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 64)

Abstract

It is now over 20 years since dendritic cells (DC) were first identified in and isolated from the spleens of mice (1,2) and they continue to be a much-studied population. Only a small proportion of spleen cells are DC, but the large size of the organ means that useful numbers of DC can still be purified. In recent years the ability to grow cells with the phenotypic and functional properties of DC from bone marrow progenitors has opened new avenues of research. However, the relationship of cells grown in this way to DC populations in vivo is unknown and the need remains to study DC present in tissues.

Keywords

Dendritic Cell Spleen Cell Dendritic Cell Subset Immunomagnetic Bead Normal Mouse Serum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Andrew J. Stagg
    • 1
  • Fiona Burke
    • 1
  • Suzanne Hill
    • 2
  • Stella C. Knight
    • 1
  1. 1.Antigen Presentation Research GroupImperial College School of Medicine, Northwick Park HospitalLondonUK
  2. 2.Cantab PharmaceuticalsCambridgeUK

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