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Applications for Green Fluorescent Protein in Cell Signaling

  • Paul Brennan
  • Emmanuelle Astoul
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 60)

Abstract

The discovery of green fluorescent protein (GFP) as a naturally fluorescing protein and its subsequent modification to allow easy detection with standard fluorescent equipment such as the fluorescence-activated cell sorter (FACS) has revolutionized our ability to detect the presence of transfected proteins and to determine their location in whole cells and in organisms. GFP was found in the bioluminescent jellyfish Aequorea (1,2). It has since been modified to change its excitation and emision (3, 4, 5). Expression vectors are now supplied commercially that allow proteins to be tagged easily (6,7). Furthermore, it is a small protein, only 29 kDa, and the DNA encoding it can be easily modified using polymerase chain reaction to introduce any restriction sites for any subcloning work required.

Keywords

Green Fluorescent Protein Cell Electroporation Cell Line RBL2H3 Green Fluorescent Protein Expression Vector Zeiss Laser Scan 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2001

Authors and Affiliations

  • Paul Brennan
    • 1
  • Emmanuelle Astoul
    • 2
  1. 1.Department of MedicineUniversity of Wales College of MedicineWales, UK
  2. 2.Lymphocyte Activation Laboratory, Imperial Cancer Research FundLondonUK

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