Intrathymic Injection for Analysis of T-Cell Progenitor Activity

  • Libuse Jerabek
  • Irving L. Weissman
Part of the Methods in Molecular Medicine book series (MIMM, volume 63)


Within our field, improvement in fluorescence-activated cell sorting (FACS) and molecular technologies has led to various types of correlative studies that imply the developmental sequence and subsequent emigration of thymic-lymphocyte subsets. Unfortunately, the implied conclusions are often accepted unequivocally by most of the immunology community. In fact, direct demonstration of precursor progeny relationships by specific cell marking within the thymus, or specific delivery of purified cells at a particular stage of isolation back into the thymus, are the only methods that reproducibly identify cell stages and intermediates (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11).


Rubber Band Amyl Alcohol Wound Clip Thymic Lobe Tribromoethyl Alcohol 
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Copyright information

© Humana Press Inc., Totowa, NJ 2002

Authors and Affiliations

  • Libuse Jerabek
    • 1
  • Irving L. Weissman
    • 1
  1. 1.Stanford University School of MedicineStanford

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