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Celiac Disease pp 163-173 | Cite as

Organ Culture of Rectal Mucosa

In Vitro Challenge with Gluten in Celiac Disease
  • Giuseppe Mazzarella
  • Francesco Paparo
  • Maria Maglio
  • Riccardo Troncone
Part of the Methods in Molecular Medicine book series (MIMM, volume 41)

Abstract

Celiac disease is sustained by an immunological process that mainly affects the jejunal mucosa (1). Nonetheless, jejunum is not the only site of the gastrointestinal tract that is involved in celiac disease. In recent years, Ensari and colleagues (2,3), by using immunohistochemical analysis and computerized image analysis for numerical quantitation, have significantly contributed to a definitive and clear demonstration of a celiac disease-associated “proctitis,” and its gluten dependence. Morphometry has shown increased populations of plasma cells, lymphocytes, and mast cells in the rectal mucosa of untreated patients, with these changes being reverted, with the sole exception of mast cells, by dietary treatment (2). The immunohistochemical approach has demonstrated highly significant increases in CD3+ and γδ+ lymphocytes within both the lamina propria and the epithelium. Mononuclear cells, both lymphocytes (CD3+) and macrophages (CD68+) expressing interleukin-2 (IL-2) receptors (CD25+), have been found to be increased in the lamina propria, usually immediately below the basal lamina. Enterocytes have been noted to be positive for major histocompatibility complex class II display, a pattern usually absent in normal colon. Furthermore, increased expression of vascular cell adhesion molecule-1 (VCAM-1) molecules in the rectal mucosa of untreated, compared to either treated celiac rectum or control mucosae, has been reported (3). As a whole, these data suggest, analogously to jeunum, an ongoing T-cell-dependent, cell-mediated immune response in the rectal mucosa.

Keywords

Celiac Disease Major Histocompatibility Complex Class Lamina Propria Rectal Mucosa Normal Rabbit Serum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Giuseppe Mazzarella
    • 1
  • Francesco Paparo
    • 2
  • Maria Maglio
    • 2
  • Riccardo Troncone
    • 2
  1. 1.Istituto di Scienze dell’Alimentazione CNRAvellinoItaly
  2. 2.Department of PediatricsUniversity Federico IINaplesItaly

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