Measurement of Sulfate in Mucins

  • Mathew J. Harrison
  • Nicolle H. Packer
Part of the Methods in Molecular Biology™ book series (MIMB, volume 125)


The sulfation of the terminal sugar residues of mucins is a common and extensive posttranslational event that greatly influences the ultimate viscoelastic properties of mucin. Highly sulfated and/or sialylated mucins comprise a considerable proportion of the mucous layers of the gastrointestinal, respiratory, and reproductive tracts, and have been demonstrated to be associated with some pathological conditions and patho-genesis (1, 2, 3, 4). The precise biological roles for glycan sulfation are largely unknown; however, several groups have demonstrated discrete biological roles for specific instances of glycan sulfation of non-mucin glycoproteins. These roles include the control of the circulatory half-life of human luteinizing hormone, symbiotic interactions of leguminous plants and nitrogen-fixing bacteria, and the targeting of lymphocytes to lymph nodes (5).


Inorganic Sulfate Curve Gradient Sulfate Peak Glycan Sulfation Human Luteinizing Hormone 
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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Mathew J. Harrison
    • 1
  • Nicolle H. Packer
    • 2
  1. 1.Macquarie University Centre for Analytical Biotechnology, School of Biological SciencesMacquarie UniversitySydneyAustralia
  2. 2.Proteome Systems Ltd.SydneyAustralia

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