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Measurement of Sulfate in Mucins

  • Mathew J. Harrison
  • Nicolle H. Packer
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 125)

Abstract

The sulfation of the terminal sugar residues of mucins is a common and extensive posttranslational event that greatly influences the ultimate viscoelastic properties of mucin. Highly sulfated and/or sialylated mucins comprise a considerable proportion of the mucous layers of the gastrointestinal, respiratory, and reproductive tracts, and have been demonstrated to be associated with some pathological conditions and patho-genesis (1, 2, 3, 4). The precise biological roles for glycan sulfation are largely unknown; however, several groups have demonstrated discrete biological roles for specific instances of glycan sulfation of non-mucin glycoproteins. These roles include the control of the circulatory half-life of human luteinizing hormone, symbiotic interactions of leguminous plants and nitrogen-fixing bacteria, and the targeting of lymphocytes to lymph nodes (5).

Keywords

Inorganic Sulfate Curve Gradient Sulfate Peak Glycan Sulfation Human Luteinizing Hormone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Mathew J. Harrison
    • 1
  • Nicolle H. Packer
    • 2
  1. 1.Macquarie University Centre for Analytical Biotechnology, School of Biological SciencesMacquarie UniversitySydneyAustralia
  2. 2.Proteome Systems Ltd.SydneyAustralia

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