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Measurement of Calcium Mobilization Responses in Killer Cell/Target Conjugates by FACS Analysis

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Part of the Methods in Molecular Biology book series (MIMB,volume 121)

Abstract

The interaction between killer cells and target cells can be dissected into three steps. First, the two cells form random conjugates mediated by nonspecific interactions between adhesion molecules (1). Second, specific recognition results in the activation of signal transduction pathways coupled to activating receptors and accessory molecules (2). This leads to the third step consisting of perforin and/or fas-ligand mediated delivery of the lethal hit (3). Lethal hit delivery requires a short time; as a consequence killer cells can recycle and kill multiple targets (4).

Keywords

  • Conjugate Formation
  • Cell Culture Incubator
  • Equipment Manual
  • Consequence Killer Cell
  • Flow Cytometry Standard

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  • DOI: 10.1385/1-59259-044-6:305
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References

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© 1999 Humana Press Inc.

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Valitutti, S., Dessing, M. (1999). Measurement of Calcium Mobilization Responses in Killer Cell/Target Conjugates by FACS Analysis. In: Campbell, K.S., Colonna, M. (eds) Natural Killer Cell Protocols. Methods in Molecular Biology, vol 121. Humana Press. https://doi.org/10.1385/1-59259-044-6:305

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  • DOI: https://doi.org/10.1385/1-59259-044-6:305

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-683-3

  • Online ISBN: 978-1-59259-044-5

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