Preparation of RNA Dot Blots

  • Rachel Hodge
Part of the Springer Protocols Handbooks book series (SPH)


RNA dot hybridizations were first described by Kafatos et al. (1). These hybridizations allow rapid analysis of mRNA expression and are particularly useful in the initial characterization of clones derived from differentially expressed genes. Where accurate quantification of transcription is necessary, or many samples have to be handled, filtration manifold systems are available such as the Millipore MilliBlot system (Bedford, MA), which uses a vacuum source to transfer nucleic acid to filter.


Fume Hood Diethyl Pyrocarbonate Incubation Solution Hair Dryer Saran Wrap 
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  1. 1.
    Kafatos, F. C., Jones, C. W., and Efstratiadis, A. (1979) Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acid Res. 7, 1541–1552.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Rachel Hodge
    • 1
  1. 1.Department of BotanyUniversity of LeicesterLeicesterUK

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