Rapid Amplification of cDNA Ends
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Rapid amplification of cDNA ends (RACE) amplifies either terminal of a cDNA, even where they have unknown sequence (1,2). RACE only requires knowledge of a short sequence within the mRNA of interest. It is often used for cloning the remainder of incomplete cDNAs.
KeywordsStructural RNAs General Primer Restriction Endonuclease Site Terminal Transferase cDNA Synthesis Primer
- 7.Bertioli, D. J. and Burrows, P. R. (1995) A simple method for the amplification of cDNA ends from small amounts of tissue. Methods Mol. Cell. Biol., in press.Google Scholar
- 9.Sambrook, J., Fritsch, E. F., and Maniatis, T. (eds.) (1989) Molecular Cloning: A Labora tory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar