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Isolation of Fungal Nucleic Acids

  • Surapareddy Sreenivasaprasad
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The last 10 years have witnessed a growing interest in fungal molecular biology and, in a number of systems, both DNA and RNA technologies have been applied to address various aspects of fungal biology. Consequently, considerable progress has been made in the use of molecular markers, i.e., restriction fragment length polymorphisms (1), fingerprints (2), polymerase chain reaction (PCR) amplification profiles (3), amplified fragment length polymorphisms (4), and sequence data (5) for fungal systematics, phy-logeny, ecology, epidemiology and population dynamics (6,7). This has led to downstream applications such as the use of probes and primers for diagnosis of pathogenic and symbiotic fungi. Moreover, from a number of fungi, genes regulating development and differentiation processes have been isolated and are being characterized (8,9). Transformation—the ability to introduce the DNA of interest into a cell—has also been achieved with a number of fungi (10). These developments have opened up the possibility of genetic manipulations in fungi of agricultural and biotechnological importance.

Keywords

Amplify Fragment Length Polymorphism Fume Hood Isoamyl Alcohol Standard Saline Citrate Fungal Biology 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Surapareddy Sreenivasaprasad
    • 1
  1. 1.Department of Plant Pathology and MicrobiologyHorticulture Research InternationalWellesbourneUK

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