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Bacterial DNA Extraction for Polymerase Chain Reaction and Pulsed-Field Gel Electrophoresis

  • Elisabeth Chachaty
  • Patrick Saulnier
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Some polymerase chain reaction (PCR) applications such as gene detection or typing (1,2) require little purified DNA and may be performed with crude bacterial extracts. Many methods have been described for this procedure ( Chapter 6). Some of them are straightforward and consist of simply boiling bacterial cells in water. However, PCR results obtained with such preparations may be erratic. The method described in this chapter (adapted from ref. 2) works well with Gram-positive bacteria such as staphylococci, enterococci, and Clostridium difficile.

Keywords

Clostridium Difficile Polymerase Chain Reaction Result Polymerase Chain Reaction Mixture Digestion Solution Digestion Step 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Elisabeth Chachaty
    • 1
  • Patrick Saulnier
    • 1
  1. 1.Service de Microbiologie MédicalInstitut Gustave-RoussyVillejuifFrance

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