Extraction and Purification of Plasmid DNA
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Plasmids may be isolated by a variety of methods many of which rely on the differential denaturation and reannealing of plasmid DNA compared to chromosomal DNA. Many of these are rapid, small-scale “minipreps” that may be used effectively for plasmid analysis and further manipulation. The methods differ primarily in the means by which cells are lysed.
KeywordsLuria Bertani Sterile Tube Lysis Solution Microfuge Tube Isoamyl Alcohol
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