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Extraction and Purification of Plasmid DNA

  • Craig Winstanley
  • Ralph Rapley
Protocol
  • 539 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Plasmids may be isolated by a variety of methods many of which rely on the differential denaturation and reannealing of plasmid DNA compared to chromosomal DNA. Many of these are rapid, small-scale “minipreps” that may be used effectively for plasmid analysis and further manipulation. The methods differ primarily in the means by which cells are lysed.

Keywords

Luria Bertani Sterile Tube Lysis Solution Microfuge Tube Isoamyl Alcohol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Birnboim, H. C. and Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7, 1513–1523.PubMedCrossRefGoogle Scholar
  2. 2.
    Holmes, D. S. and Quigley, M. (1981) A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 114, 193–197.PubMedCrossRefGoogle Scholar
  3. 3.
    Maniatis, T., Fritsch, E. F., and Sambrook, J. (eds.) (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 368,369.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Craig Winstanley
    • 1
  • Ralph Rapley
    • 2
  1. 1.Department of Medical MicrobiologyUniversity of LiverpoolLiverpoolUK
  2. 2.Department of BiosciencesUniversity of HertfordshireHatfieldUK

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