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Primer Extension Analysis of mRNA

  • Maggie Walmsley
  • Mark Leonard
  • Roger Patient
Protocol
  • 124 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Primer extension is a relatively quick and convenient means by which gene transcription can be monitored. The technique can be used to determine accurately the site of transcription initiation or to quantify the amount of cap site-specific message produced.

Keywords

Premature Termination Isoamyl Alcohol Primer Extension Analysis Prime Excess Autoclave Water 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Blumberg, D. (1987) Creating a ribonuclease-free environment, in Methods in Enzymology, vol. 152 (Berger, S. L. and Kimmel, A. R., eds.), Academic, London, New York, pp. 20–24.Google Scholar
  2. 2.
    Thomas, M., White, R. L., and Davis, R. W. (1976) Hybridisation of RNA to double-stranded DNA. Proc. Natl. Acad. Sci. USA 73, 2294–2298.PubMedCrossRefGoogle Scholar
  3. 3.
    Britten, R. J. and Davidson, E. H. (1985) Hybridisation strategy, in Nucleic Acid Hybridisation—A Practical Approach (Hames, B. D. and Higgins, S. J., eds.), IRL, Oxford, Washington, DC, pp. 3–15.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Maggie Walmsley
    • 1
  • Mark Leonard
    • 1
  • Roger Patient
    • 1
  1. 1.Developmental Biology Research Centre, Kings CollegeUniversity of LondonLondonUK

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