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Nonradioactive Oligonucleotide Probe Labeling

  • Sue Fowler
  • Ian Durrant
Protocol
  • 120 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Nonradioactive oligonucleotide probes are increasingly being used in various applications, for example, polymerase chain reaction (PCR) product detection, in situ hybridization and colony and plaque screening (1). Oligonucleotide probes are short, defined sequences, 15–50 bases long, that are relatively easy to produce. As the base composition of oligonucleotide probes dictates the hybridization properties (2), probes can be designed to discriminate between perfectly matched and mismatched target sequences.

Keywords

Oligonucleotide Probe Amersham Pharmacia Biotech Standard Saline Citrate Stringency Wash Trityl Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Sue Fowler
    • 1
  • Ian Durrant
    • 1
  1. 1.Research and DevelopmentAmersham Pharmacia Biotech, Ltd.UK

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