Biochemical Measurement of Cholinesterase Activity

  • Stephanie Padilla
  • T. Leon Lassiter
  • Deborah Hunter
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 22)


The cholinesterases (acetylcholinesterase and butyrylcholinesterase)

In this chapter, cholinesterase will be used to refer to both enzymes together (i.e., total cholinesterase), whereas acetylcholinesterase or butyrylcholinesterase will be used when referring to the specific esterase.


Cholinesterase Activity Acetylcholinesterase Activity Microtiter Plate Reader Acetylthiocholine Iodide Radiometric Assay 
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  1. 1.
    Silver, A., ed. (1974) The Biology of Cholinesterases, North Holland, Amsterdam, The Netherlands.Google Scholar
  2. 2.
    Kutty, K. M. (1980) Review: biological function of cholinesterase. Clin. Biochem. 13, 239–242.PubMedCrossRefGoogle Scholar
  3. 3.
    Drews, U. (1975) Cholinesterase in embryonic development. Prog. Histochem. Cytochem. 7, 3–52.Google Scholar
  4. 4.
    Kristt, D. A. (1983) Acetylcholinesterase in the ventrobasal thalamus: transience and patterning during ontogenesis. Neuroscience 10, 923–939.PubMedCrossRefGoogle Scholar
  5. 5.
    Layer, P. G. (1991) Cholinesterases during development of the avian nervous system. Cell. Mol. Neurobiol. 11, 7–34.PubMedCrossRefGoogle Scholar
  6. 6.
    Dass, P., Mejia, M., Landes, M., Jones, R., Stuart, B., and Thyssen, J. (1994) Cholinesterase: review of methods. Clin. Chem. 34, 135–157.Google Scholar
  7. 7.
    Ellman G. L., Courtney, D. K., Anders, V., and Featherstone, R. M. (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7, 88–95.PubMedCrossRefGoogle Scholar
  8. 8.
    Aldridge, W. N. and Reiner, E. (1975) Enzyme Inhibitors as Substrates: Interaction of Esterases with Esters of Organophosphorus and Carbamic Acids. North-Holland Publishing Co., Amsterdam, The Netherlands.Google Scholar
  9. 9.
    Johnson, C. D. and Russell, R. L. (1975) A rapid, simple radiometric assay for cholinesterase, suitable for multiple determinations. Analyt. Biochem. 64, 229–238.PubMedCrossRefGoogle Scholar
  10. 10.
    Wilson, B. W., Padilla, S., Henderson, J. D., et al. (1996) Factors in standardizing automated cholinesterase assays. J. Toxicol. Environ. Health 48, 187–195.PubMedCrossRefGoogle Scholar
  11. 11.
    Hunter, D. L., Marshall, R. S., and Padilla, S. (1997) Automated instrument analysis of cholinesterase activity in tissues from carbamate-treated animals: a cautionary note. Tox. Methods 7, 43–53.CrossRefGoogle Scholar
  12. 12.
    Willig, S., Dass, P. D., and Padilla, S. (1996) Validation of the use of 6,6′-dithiodinicotinic acid as a chromogen in the Ellman method for cholinesterase determinations. Vet. Hum. Toxicol. 38, 249–253.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Stephanie Padilla
    • 1
  • T. Leon Lassiter
    • 2
  • Deborah Hunter
    • 1
  1. 1.Neurotoxicology DivisionUS Environmental Protection AgencyResearch Triangle Park
  2. 2.Curriculum in ToxicologyUniversity of North CarolinaChaptel Hill

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