Recovery of Mycoplasmas from Animals

  • Robin Nicholas
  • Samantha Baker
Part of the Methods in Molecular Biology™ book series (MIMB, volume 104)


Of the 30 or so mollicute species that have been isolated from small and large ruminants, only a handful have been shown to cause disease in their own right. This chapter will concentrate on methods for sampling, transporting, and isolating those pathogenic mycoplasmas, including Mycoplasma mycoides subsp. mycoides small colony (SC), Mycoplasma capricolum subsp. capripneumoniae, Mycoplasma bovis, Mycoplasma agalactiae, and M. m. mycoides large colony (LC), which cause economically important diseases, such as contagious bovine pleuropneumonia (CBPP), contagious caprine pleuropneumonia (CCPP), and contagious agalactia. Although DNA amplification techniques are being used with ever-increasing frequency for the detection and identification of mycoplasmas (see  Chapters 18 20), the isolation of these organisms by conventional techniques is still required by most national and international authorities to confirm disease outbreaks.


Pleural Fluid Transport Medium Small Colony Large Ruminant Fresh Broth 
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Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • Robin Nicholas
    • 1
  • Samantha Baker
    • 1
  1. 1.Bacteriology DepartmentCentral Veterinary LaboratorySurreyUK

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