Neem and Related Natural Products

  • Murray B. Isman
Part of the Methods in Biotechnology book series (MIBT, volume 5)


Although botanical insecticides once held a position of importance in the grower’s arsenal of plant protection products, they were almost completely displaced in most industrialized countries by synthetic insecticides in the 1950s and 1960s. However, increasing documentation of the negative environmental and health impacts of synthetic neurotoxic insecticides and increasingly stringent government regulation of pesticides has resulted in renewed interest in the development and use of botanical pest management products In spite of this interest, there remain only a handful of botanical insecticides in use in North America and Europe, with few new products on the threshold of commercialization (1, 2, 3).


  1. 1.
    Isman, M B (1994) Botanical insecticides Pesticide Outlook 5, 26–31Google Scholar
  2. 2.
    Isman, M B (1995) Leads and prospects for the development of new botanical insecticides Rev Pestic Toxicol 3, 1–20Google Scholar
  3. 3.
    Isman, M B (1997) Neem and other botanical insecticides banlers to commercialization Phytoparasitica 25, 339–344.CrossRefGoogle Scholar
  4. 4.
    Schmutterer, H, ed (1995) The Neem Tree VCH, Weinheim, GermanyGoogle Scholar
  5. 5.
    Lowery, D. T and Isman, M. B (1995) Toxicity of neem to natuial enemies of aphids Phytoparasitica 23, 297–306CrossRefGoogle Scholar
  6. 6.
    Naumann, K and Isman, M B. (1996) Toxicity of neem (Azadirachta indica A Juss) seed extracts to larval honeybees and estimation of dangeis from field applications Am Bee J 136, 518–520Google Scholar
  7. 7.
    van Beek, T A and de Groot, A (1986) Terpenoid antifeedants Parti An overview of terpenoid antifeedants of natural origin Recueil des Travaux Chimiques des Pays-Bas 105, 513–527CrossRefGoogle Scholar
  8. 8.
    O′Reilly, J (1993) Ginkgo biloba-cultivation, extraction and therapeutic use of the extract, in Phytochemistry and Agriculture (van Beek, T. A and Bretelei, H, eds), Clarendon, Oxford, pp 253–270Google Scholar
  9. 9.
    Ahn, Y J, Kwon, M, Park, H. M, and Han, C G (1997) Potent insecticidal activity of Ginkgo biloba derived tnlactone terpenes against Nilaparvata lugens, in Phytochemicals for Pest Control (Hedin, P A, Holhngworth, R M, Masler, E P, Miyamoto, J., and Thompson, D G, eds.), American Chemical Society, Washington, DC, pp 90–105.CrossRefGoogle Scholar
  10. 10.
    Ratnayake, S, Rupprecht, J K, Potter, W. M, and McLaughlin, J L (1991) Evaluation of the pawpaw tree, Asimina triloba (Annonaceae), as a commercial source of the pesticidal annonaceous acetogenins, in New Crops (Janick, J and Simon, J E, eds.), Wiley, New York, pp. 644–648Google Scholar
  11. 11.
    Marr, K L and Tang, C. S. (1992) Volatile insecticidal compounds and chemical variability of Hawaiian Zanthoxylum (Rutaceae) species Bwchem Syst Ecol 20, 209–217CrossRefGoogle Scholar
  12. 12.
    Satasook, C, Isman, M B, Ishibashi, F, Medbury, S, Winyachitra, P., and Towers, G H N (1994) Insecticidal bioactivity of crude extracts of Aglaia species (Meliaceae) Bwchem Syst Ecol 22, 121–127CrossRefGoogle Scholar
  13. 13.
    Rajasekaran, T, Pereira, J, Ravishankar, G A., and Venkataraman, L V (1996) Repellency of callus derived pyrethrins to mosquito Culex qmnquefasciatus Say and red flour beetle Tribolium castaneum Herbst Int Pest Control 38, 155–159Google Scholar
  14. 14.
    Morgan, E. D., van der Esch, S A., Jarvis, A P, Maccioni, O., Giagnacovo, G, and Vitale, F (1996) Production of natural insecticides from Azadirachta species by tissue culture. Abstract, International Neem Conference, Lawes, Australia.Google Scholar
  15. 15.
    Khambay, B P. S. and O′Connor, N (1993) Progress in developing insecticides from natural compounds, in Phytochemistty and Agriculture (van Beek, T A and Breteler, H, eds), Clarendon, Oxford, pp 40–61Google Scholar
  16. 16.
    Sharma, R N (1984) Development of pest control agents from plants a comprehensive working strategy, in Natural Pesticides from the Neem Tree and Other Tropical Plants (Schmutterer, H and Ascher, K R S, eds), GTZ, Eschborn, pp 551–563Google Scholar
  17. 17.
    Escoubas, P., Lajide, L, and Mizutani, J (1994) Insecticidal and antifeedant activities of plant compounds, potential leads for novel pesticides, in Natural and Engineered Pest Management Agents (Hedin, P. A., Menn, J. J., and Holhngworth, R M, eds), American Chemical Society, Washington, DC, pp 162–171Google Scholar
  18. 18.
    Alkofahi, A., Rupprecht, J. K., Anderson, J E., McLaughlin, J L, Mikolajczak, K L, and Scott, B. A (1989) Search for new pesticides from higher plants, in Insecticides of Plant Origin (Arnason, J T, Philogene, B J R, and Morand, P, eds.), American Chemical Society, Washington, DC, pp 25–43CrossRefGoogle Scholar
  19. 19.
    Cepleanu, F, Hamburger, M O, Sordat, B, Msonthi, J D, Gupta, M P, Saadou, M, and Hostettman, K (1994) Screening of tropical medicinal plants for mollus-cicidal, larvicidal, fungicidal and cytotoxic activities and brine shrimp toxicity Int J Pharmacog 32, 294–307.CrossRefGoogle Scholar
  20. 20.
    Bomford, M. K and Isman, M. B. (1996) Desensitization of fifth instar Spodoptera litura (Lepidoptera Noctuidae) to azadirachtin and neem Entomol Exp Appl 81, 307–313CrossRefGoogle Scholar
  21. 21.
    Isman, M. B. (1993) Growth inhibitory and antifeedant effects of azadirachtin on six noctuids of regional economic importance Pesticide Sci 38, 57–63CrossRefGoogle Scholar
  22. 22.
    Champagne, D. E., Isman, M B, and Towers, G. H. N (1989) Insecticidal activity of phytochemicals and extracts of the Mehaceae, in Insecticides of Plant Origin (Arnason, J. T., Philogene, B. J. R., and Morand, P., eds.), American Chemical Society, Washington, DC, pp 95–109CrossRefGoogle Scholar
  23. 23.
    Lowery, D. T and Isman, M B. (1993) Antifeedant activity of extracts from neem, Azadirachta indica, to strawberry aphid, Chaetosiphon fragaefolu J Chem Ecol 19, 1761–1773.CrossRefGoogle Scholar
  24. 24.
    Isman, M B, Proksch, P, and Yan, J-W. (1987) Insecticidal chromenes from the Asteraceae: structure-activity relations. Entomol Exp Appl 43, 87–93Google Scholar
  25. 25.
    Sims, M. (1981) Liquid carbon dioxide extraction of pyrethnns US Patent No 4,281,171Google Scholar
  26. 26.
    Lowery, D. T and Isman, M B (1994) Insect growth regulating effects of neem extract and azadirachtin on aphids Entomol Exp Appl 72, 77–84Google Scholar
  27. 27.
    Arnason, J. T., Philogene, B. J. R, Morand, P., Imrie, K., Iyengar, S., Duval, F., et al (1989) Naturally occurring and synthetic thiophenes as photoactivated insecticides, in Insecticides of Plant Origin (Arnason, J T, Philogene, B J R, and Morand, P, eds), American Chemical Society, Washington, DC, pp. 164–172CrossRefGoogle Scholar
  28. 28.
    Mendel, M J., Alford, A. R., and Bentley, M. D. (1991) A comparison of the effects of limonin on Colorado potato beetle, Lephnotarsa decemhneata, and fall armyworm, Spodoptera frugiperda, larval feeding. Entomol Exp. Appl 58, 191–194.Google Scholar
  29. 29.
    Spollen, K. M. and Isman, M. B. (1996) Acute and sublethal effects of a neem insecticide on the commercial biocontrol agents Phytoseiulus persimihs and Amblyseius cucumens (Acan Phytosendae), and Aphidoletes aphidimyza (Rondani) (Diptera. Cecidomyiidae) J. Econ. Entomol 89, 1379–1386Google Scholar
  30. 30.
    Naumann, K, Curne, R. W, and Isman, M. B. (1994) Evaluation of the repellent effects of a neem insecticide on foraging honey bees and other pollinators Can Entomol 126, 225–230.CrossRefGoogle Scholar
  31. 31.
    Ascher, K R S, Schmutterer, H., Zebitz, C. P. W., and Naqvi, S. N H (1995) The Persian lilac or chinaberry tree: Melia azedarach L, in The Neem Tree (Schmutterer, H, ed), VCH, Weinheim, pp. 605–642Google Scholar
  32. 32.
    Chiu, S-F. (1995) Melia toosendan Sieb. & Zucc, in The Neem Tree (Schmutterer, H, ed), VCH, Weinheim, pp. 642–646.Google Scholar
  33. 33.
    Moeschler, H. F., Pfuger, W., and Wendlisch, D. (1987) Pure annonin and a process for the preparation thereof. US Patent No. 4,689,323Google Scholar
  34. 34.
    Mikolajczak, K L, McLaughlin, J. L., and Rupprecht, J. K. (1988) Control of pests with annonaceous acetogenins. US Patent No. 4,721,727.Google Scholar
  35. 35.
    McLaughlin, J. L., Zeng, L, Oberlies, N H, Alfonso, D, Johnson, H A, and Cummings, B. A. (1997) Annonaceous acetogenins as new natural pesticides. recent progress, in Phytochemicals For Pest Control (Hedin, P. A., Hollingworth, R. M, Masler, E. P, Miyamoto, J, and Thompson, D G, eds), American Chemical Society, Washington, DC, pp. 117–133.CrossRefGoogle Scholar
  36. 36.
    Pittarelh, G W, Buta, J. G., Neal, J W, Jr., Lusby, W. R, and Waters, R M (1993) Biological pesticide derived from Nicotiana plants US Patent No. 5,260,281Google Scholar
  37. 37.
    Rembold, H and Mwangi, R W. (1995) Melia volkensn Gurke, in The Neem Tree (Schmutterer, H., ed), VCH, Weinheim, Germany, pp. 647–652.Google Scholar
  38. 38.
    Murray, K. D., Alford, A. R., Groden, E., Drummond, F. A., Storch, R H., Bentley, M. D., and Sugathapala, P. M. (1993) Interactive effects of an antifeedant used with Bacillus thuringiensis var. san diego delta endotoxin on Colorado potato beetle (Coleoptera: Chrysomehdae). J Econ Entomol 86, 1793–1801Google Scholar
  39. 39.
    Wink, M. (1993) Production and application of phytochemicals from an agricultural perspective, in Phytochemicals and Agriculture (van Beek, T A. and Breteler, H, eds), Clarendon, Oxford, pp 171-213.Google Scholar
  40. 40.
    Vollinger, M. (1995) Studies of the probability of development of resistance of Plutella xylostella to neem products, in The Neem Tree (Schmutterer, H., ed), VCH, Weinheim, Germany, pp. 477–483Google Scholar
  41. 41.
    Feng, R. and Isman, M. B. (1995) Selection for resistance to azadirachtin in the green peach aphid, Myzus persicae Experientia 51, 831–833CrossRefGoogle Scholar

Copyright information

© Humana Press, Totowa, NJ 1999

Authors and Affiliations

  • Murray B. Isman
    • 1
  1. 1.Department of Plant ScienceUniversity of Brtttsh ColumbiaVancouverCanada

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