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Pulsed Field Gel Electrophoresis for Mycobacteria

  • Wolfgang J. Philipp
  • Stephen Gordon
  • Amalio Telenti
  • Stewart T. Cole
Part of the Methods in Molecular Biology™ book series (MIMB, volume 101)

Abstract

The introduction of pulsed field gel electrophoresis (PFGE) was a major breakthrough in the field of genome research (1), allowing the analysis of whole procaryotic and eucaryotic chromosomes. This became possible by a combination of new techniques for the extraction of intact chromosomal DNA in agarose blocks and electrophoresis using varying electrical fields.

Keywords

Bovine Serum Albumine Ethidium Bromide Solution Lowenstein Jensen Mycobacterium Kansasii Mycobacterial Genome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1998

Authors and Affiliations

  • Wolfgang J. Philipp
    • 1
  • Stephen Gordon
    • 2
  • Amalio Telenti
    • 1
  • Stewart T. Cole
    • 3
  1. 1.Institute of Medical MicrobiologyUniversity of BerneBerneSwitzerland
  2. 2.Unitè de Gènètique Molèculaire BactèrienneInstitut PasteurParisFrance
  3. 3.Unite de Genetique Mycobacterienne, Departement de Bacteriologie et MycologieInstitut PasteurParisFrance

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