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Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 101)

Abstract

Mobile generic elements are discrete DNA fragments encoding their own transposltlon functions, which enable them to move from one site in the DNA to another, independently of host-recombination functions (such as RecA). Recombination by transposition occurs without homology between the sequences of the transposon and the target sequence (1). Moreover, most of these elements do not exhibit a strong specificity for the target-site sequence, thus allowing these elements to be widely used for mutagenesis in bacteria (2).

Keywords

  • Mycobacterium Smegmatis
  • Transposition Event
  • Electroporation Cuvets
  • Kanamycin Sulfate
  • Mycobacterium Fortuitum

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© 1998 Humana Press Inc., Totowa, NJ

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Pérez, E. et al. (1998). Tn611 Transposon Mutagenesis in Mycobacterium smegmatis Using a Temperature-Sensitive Delivery System. In: Parish, T., Stoker, N.G. (eds) Mycobacteria Protocols. Methods in Molecular Biology™, vol 101. Humana Press. https://doi.org/10.1385/0-89603-471-2:187

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  • DOI: https://doi.org/10.1385/0-89603-471-2:187

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-471-6

  • Online ISBN: 978-1-59259-576-1

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