mRNA Abundance and Half-Life Measurements
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The expression of many genes is regulated at multiple levels. For most genes, the predominant control is at transcription, and this is usually inferred from changes in mRNA levels that respond to specific stimuli. However, changes in mRNA stability may contribute to observed variation in mRNA abundance, and many such changes in mRNA stability probably remain undetected. The relative contributions of posttranscriptional regulatory circuits can only be detected by careful RNA analysis.
KeywordsmRNA Abundance Northern Analysis Filter Hybridization Fume Cupboard Morpholinopropanesulfonic Acid
- 2.Bettany, A. J. E., Moore, P. A., Cafferkey, R., Bell, L. D., Goodey, A. R., Carter, B. L. A., and Brown, A. J. P. (1989) 5′-secondary structure formation, in contrast to a short string of non-preferred codons, inhibits the translation of the pyruvate kinase mRNA in yeast. Yeast 5, 187–198.PubMedCrossRefGoogle Scholar
- 6.Sambrook, J., Fritsch, E. F., and Maniatis, T. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.Google Scholar
- 7.Lithgow, G. J. (1989) Transcription in the yeast Saccharomyces cerevisiae. PhD Thesis, University of Glasgow.Google Scholar