Multilocus Enzyme Electrophoresis

  • Patrick Boerlin
  • Jean-Claude Piffaretti
Part of the Methods in Molecular Biology™ book series (MIMB, volume 46)


Multilocus enzyme electrophoresis (MEE) has been used extensively in the past in eukaryotic, and occasionally, prokaryotic genetics. It was only recently that the usefulness of MEE in bacterial genetics, systematics, and epidemiology, has been clearly demonstrated, essentially by the pioneering work of Selander and coworkers (1, 2, 3). Since then, numerous authors have been successfully applying MEE to an increasing number of bacteria. In MEE, cellular extracts are submitted to gel electrophoresis under nondenaturing conditions and enzymes are detected with specific staining methods (4,5).


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Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • Patrick Boerlin
    • 1
  • Jean-Claude Piffaretti
    • 2
  1. 1.Institut de MicrobiologieLausanneSwitzerland
  2. 2.Instituto Cantonale BatteriosierologicoLuganoSwitzerland

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