Cell Culture Phototoxicity Test

  • Paul A. Duffy
Part of the Methods in Molecular Biology™ book series (MIMB, volume 43)


Human A431 cells and mouse 3T3 cells are exposed in culture to UV light both in the presence and absence of test compound. Phototoxicity is expressed as a decrease in cell viability as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay.


Test Compound Solar Simulator Region Filter Human A431 Cell Epidermal Carcinoma Cell 
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Copyright information

© Humana Press Inc , Totowa, NJ 1995

Authors and Affiliations

  • Paul A. Duffy
    • 1
  1. 1.Zeneca PharmaceuticalsMacclesfield CheshireUK

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