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Scale-Up of Oligonucleotide Synthesis

Solution Phase
  • H. Seliger
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 20)

Abstract

Solution-phase methods have been used for the first synthesis of an internucleotidic bond (1) and, some 20 years ago, had a glorious period during the first gene synthesis. At that time, their present-day competitor, the polymer support technique, was also developed and saw some first applications to oligonucleotide preparations (2), but it took nearly 15 further years until the solid-phase methods became a serious rival to solution methods. The efficiency of the phosphoramidite chemistry, combined with the development of mechanization, brought about a dramatic change ca. 10 years ago, putting polymer-support synthesis into first place. Yet, solution methods were never completely replaced, and the following survey will testify to their vitality.

Keywords

Solution Synthesis Oligonucleotide Synthesis Polymeric Reagent Polynucleotide Phosphorylase Nucleoside Phosphate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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© Humana Press Inc., Totowa, NJ 1993

Authors and Affiliations

  • H. Seliger
    • 1
  1. 1.Polymer SectionUniversity of UlmUlmGermany

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