The Bradford Method for Protein Quantitation

  • Nicholas J. Kruger
Part of the Methods in Molecular Biology™ book series (MIMB, volume 32)


A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. An assay originally described by Bradford (1) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of biological samples (see Note 1).


Protein Standard Bradford Assay Reagent Blank Lowry Method Guanidine Hydrochloride 
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Copyright information

© Humana Press Inc., Totowa, NJ 1994

Authors and Affiliations

  • Nicholas J. Kruger
    • 1
  1. 1.Department of Plant SciencesUniversity of OxfordUK

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