• Jean de Vellis
  • Araceli Espinosa de los Monteros
Part of the Neuromethods book series (NM, volume 23)


Oligodendrocytes were first described by Robertson in 1899 as small cells with a few processes of variable length. In 1921, del Rio Hortega published a detailed histological description of these cells and coined the descriptive word “oligodendrocyte.” The localization of these cells along axonal tracts, around neuronal cell bodies, and in proximity to capillaries served as the basis for del Rio Hortega’s classification of oligodendrocytes into three subgroups: interfascicular, perineural satellite, and perivascular. The heterogeneity of oligodendrocytes has also been observed at the ultrastructural level and by the use of immunocytochemical markers (for review and ref. see Espinosa de los Monteros and de Vellis, 1988; Goldman and Vaysse, 1991). However, there is no evidence for more than one oligodendrocyte lineage (Skoff and Knapp, 1991).


Glial Fibrillary Acidic Protein Myelin Basic Protein Mature Oligodendrocyte Oligodendroglial Cell Oligodendrocyte Differentiation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Arenander A. and de Vellis J. (1989) Development of the nervous system, in Basic Neurochemistry: Molecular. Cellular and Medical Aspects (Siegel G. J. et al., eds.), Raven, New York, pp. 479–506.Google Scholar
  2. 2.
    Bhat S., Barbarese E., and Pfeiffer S. E. (1981) Requirement for nonoligodendrocyte cell signals for enhanced myelinogenic gene expression in long-term cultures of purified rat oligodendrocytes. Proc. Natl. Acad. Sci. 78, 1283–1287.PubMedCrossRefGoogle Scholar
  3. 3.
    Bhat S. and Silbergerg D. H. (1989) Isolation and culture of rat and mouse brain oligodendrocytes, in A Dissection and Tissue Culture Manual of the Nervous System (Shahar A., de Vellis J., Vernadakis A., and Haber B., eds.), Alan R. Liss, New York, pp. 145–147.Google Scholar
  4. 4.
    Bologa L., Aizenman Y., Chiappelli F., and de Vellis J. (1986) Regulation of myelin basic protein in oligodendrocytes by a soluble neuronal factor. J. Neurosci. Res. 15, 521–528.PubMedCrossRefGoogle Scholar
  5. 5.
    Bornstein M. B. and Murray M. R. (1958) Serial observations on patterns of growth, myelin formation, maintenance and degeneration in cultures of new-born rat and kitten cerebellum. J. Biophys. Biochem. Cytd. 4, 499–504.CrossRefGoogle Scholar
  6. 6.
    Breen G. A. M. and de Vellis J. (1974) Regulation of glycerol phosphate dehydrogenase by hydrocortisone in dissociated rat cerebral cell culture. Dev. Biol. 41, 255–266.PubMedCrossRefGoogle Scholar
  7. 7.
    Breen G. A. M. and de Vellis J. (1975) Regulation of glycerol phosphate dehydrogenase by hydrocortisone in rat brain explants. Exp. Cell Res. 91, 159–169.PubMedCrossRefGoogle Scholar
  8. 8.
    Breen G. A. M., McGinnis J. F., and de Vellis J. (1978) Modulation of the hydrocortisone induction of glycerol phosphate dehydrogenase by N6, O2-dibutyryl cyclic AMP, norepinephrine and isobutylmethyl-xanthine in rat brain cell cultures. J. Biol. Chem. 253, 2554–2562.PubMedGoogle Scholar
  9. 9.
    Bunge M. B., Bunge R. P., and Pappas G. D. (1962) Electron microscopic demonstrations of connections between glia and myelin sheaths in the developing mammalian central nervous system. J. Cell Biol. 12, 448–453.PubMedGoogle Scholar
  10. 10.
    Cardwell M. C. and Rome L. H. (1988a) Evidence that an RGD-dependent receptor mediates the binding of oligodendrocytes to a novel ligand in a glial-derived matrix. J. Cell Biol. 107, 1541–1549.PubMedCrossRefGoogle Scholar
  11. 11.
    Cardwell M. C. and Rome L. H. (1988b) RGD-containing peptides inhibit the synthesis of myelin-like membrane by cultured oligodendrocytes. J. Cell Biol. 107, 1551–1559.PubMedCrossRefGoogle Scholar
  12. 12.
    Cameron R. S. and Rakic P. (1991) Glial cell lineage in the cerebral cortex: A review and synthesis. Glial Special Issue 4, 124–137.Google Scholar
  13. 13.
    del Rio Hortega P. (1921) Postnatal gliogenesis in the mammalian brain. Int. Rev. Cytol. 40, 281–323.Google Scholar
  14. 14.
    Espinosa de los Monteros A., Roussel G., Gensburger C., Nussbaum J. L., and Labourdette G. (1985) Precursor cells of oligodendrocytes in rat primary cultures. Dev. Biol. 108, 474–480.CrossRefGoogle Scholar
  15. 15.
    Espinosa de los Monteros A., Roussel G., and Nussbaum J.-L. (1986) A procedure for long-term culture of oligodendrocytes. Dev. Brain Res. 24, 117–125.CrossRefGoogle Scholar
  16. 16.
    Espinosa de los Monteros A. (1987) Contribution a l’etude des oligodendrocytes in vitro. Universit Louis Pasteur, Strasbourg, France, Ph.D. Thesis.Google Scholar
  17. 17.
    Espinosa de los Monteros A. and de Vellis J. (1988) Myelin basic protein and transferrin characterize different sub-populations of oligodendrocytes in rat primary glial cultures. J. Neurosci. Res. 21, 181–187.CrossRefGoogle Scholar
  18. 18.
    Espinosa de los Monteros A., Chiappelli F., Fisher R. S., and de Vellis J. (1988a) Transferrin: An early marker of oligodendrocytes in culture. Int. J. Develop. Neurosci. 6, 167–175.CrossRefGoogle Scholar
  19. 19.
    Espinosa de los Monteros A., Roussel G., Neskovic N. M., and Nussbaum J. L. (1988b) A chemically defined medium for the culture of mature oligodendrocytes. J. Neurosci. Res. 19, 202–211.CrossRefGoogle Scholar
  20. 20.
    Espinosa de los Monteros A., Pefta L. A., and de Vellis J. (1989) Does transferrin have a special role in the nervous system? J. Neurosci. Res. (New York) 24, 125–136.CrossRefGoogle Scholar
  21. 21.
    Espinosa de los Monteros A., Zhang M., Gordon M., Aymie M., and de Vellis J. (1991) Transplantation of cultured premyelinating oligodendrocytes into normal and myelin-deficient rat brain. (Submitted for publication).Google Scholar
  22. 22.
    Gard A. L. and Pfeiffer S. E. (1990) Two proliferative stages of the oligodendrocyte lineage (A2B5+04- and 04+GalC-) under different mitogenic control. Neuron 5, 615–625.PubMedCrossRefGoogle Scholar
  23. 23.
    Giulian D. (1987) Ameboid microglia as effectors in inflammation in the central nervous system. J. Neurosci. Res. 18, 155–171.PubMedCrossRefGoogle Scholar
  24. 24.
    Goldman J. E. and Vaysse P. J.-J. (1991) Tracing glial cell lineages in the mammalian forebrain. Glia 4, 149–156.PubMedCrossRefGoogle Scholar
  25. 25.
    Holmes E., Hermanson G., Cole R., and de Vellis J. (1988) Developmental expression of glial specific mRNAs in primary cultures of rat brain visualized by in situ hybridization. J. Neurosci. Res. 19, 389–396, 458–465.PubMedCrossRefGoogle Scholar
  26. 26.
    Knapp P. E., Bartlett W. P., and Skoff R. P. (1987) Cultured oligodendrocytes mimic in vivo phenotypic characteristics: Cell shape, expression of myelin-specific antigens and membrane production. Dev. Biol. 120, 356–365.PubMedCrossRefGoogle Scholar
  27. 27.
    Kumar S. and de Vellis J. (1981) Induction of lactate dehydrogenase by dibutyryl cAMP in primary cultures of central nervous tissue is an oligodendroglial marker. Dev. Brain Res. 1, 303–307.CrossRefGoogle Scholar
  28. 28.
    Kumar S. and de Vellis J. (1987) Glucocorticoid mediated functions in glial cells, in Glial Cell Receptors (Kimelberg H. K., ed.), Raven, New York, pp. 243–263.Google Scholar
  29. 29.
    Kumar S., Cole R., Chiappelli R, and de Vellis J. (1989) Differential regulation of oligodendrocyte markers by glucocorticoids: Post-transcriptional regulation of PLP, MBP and transcriptional regulation of GPDH. Proc. Natl. Acad. Sci. 86, 6807–6811.PubMedCrossRefGoogle Scholar
  30. 30.
    Labourdette G., Roussel G., Ghandour M. S., and Nussbaum J. L. (1979) Cultures from rat brain hemispheres enriched in oligodendrocyte-like cells. Brain Res. 179, 199–203.PubMedCrossRefGoogle Scholar
  31. 31.
    Labourdette G., Roussel G., and Nussbaum J. L. (1980) Oligodendroglia content of glial cell primary cultures, from newborn rat brain hemispheres, depends on the initial plating density. Neurosci. Lett. 18, 203–209.PubMedCrossRefGoogle Scholar
  32. 32.
    Leveille P. J., McGinnis J. F., Maxwell D. S., and de Vellis J. (1980) Immunocytochemical localization of glycerol-3-phosphate dehydrogenase in ratoligodendrocytes. Brain Res. 196, 287–305.PubMedCrossRefGoogle Scholar
  33. 33.
    Lu E., Brown W. J., Cole R., and de Vellis J. (1980) Ultrastructural differentiation and synaptogenesis in aggregating rotation cultures of rat cerebral cells. J. Neurosci. Res. 5, 447–463.PubMedCrossRefGoogle Scholar
  34. 34.
    Matthieu J.-M., Honegger P., Trapp B. D., Cohen S. R., Webster H. D. F. (1978) Myelination of rat brain aggregating cell cultures. Neuroscience 3, 565–572.PubMedCrossRefGoogle Scholar
  35. 35.
    Matthieu J.-M., Roch J.-M., Torch S., Tosic M., Carpano P., Insirello L., Giuffrida Stella A. M., and Honegger P. (1990) Triiodothyronine increases the stability of myelin basic protein mRNA in aggregating brain cell cultures, in Regulation of Gene Expression in the Nervous System (Giuffrida Stella A. M., de Vellis J., and Perez-Polo J. R., eds.), Wiley-Liss, New York, pp. 109–122.Google Scholar
  36. 36.
    McCarthy K. D., McGinnis J. F., and de Vellis J. (1978) Alpha-adrenergic receptor modulation of beta-adrenergic, adenosine and prostaglandin E1 increased adenosine 3′:5′-cyclic monophosphate levels in primary cultures of glia. J. Cyclic Nucleotide Res. 4, 15–26.PubMedGoogle Scholar
  37. 37.
    McCarthy, K. D. and de Vellis J. (1980) Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J. Cell Biol. 85, 890–902.PubMedCrossRefGoogle Scholar
  38. 38.
    McKinnon R. D., Matsui T., Dubois-Dalcq M., and Aaronson S. A. (1990) FBF modulates the PDGF-driven pathway of oligodendrocyte development. Neuron 5, 603–614.PubMedCrossRefGoogle Scholar
  39. 39.
    McMorris F. A. and Dubois-Dalcq M. (1988) Insulin-like growth factor 1 promotes cell proliferation and oligodendroglial commitment in rat glial progenitor cells developing in vitro. Neurosci. Res. 21, 199–209.CrossRefGoogle Scholar
  40. 40.
    Morrison R. S. and de Vellis J. (1981) Growth of purified astrocytes in a chemically defined medium. Proc. Natl. Acad. Sci. 78, 7205–7209.PubMedCrossRefGoogle Scholar
  41. 41.
    Morrison R. S. and de Vellis J. (1983) Differentiation of purified astrocytes in a chemically defined medium. Dev. Brain Res. 9, 337–345.CrossRefGoogle Scholar
  42. 42.
    Noble M. (1991) Points of controversy in the O-2A lineage: Clocks and Type-2 astrocytes. Glia 4, 157–164.PubMedCrossRefGoogle Scholar
  43. 43.
    Norton W. T. (1984) Oligodendroglia, Plenum, New York.Google Scholar
  44. 44.
    Peters A., Palay S. L., and deF. Webster H. (1976) The Fine Structure of the Nervous System: The Neurons and Supportina Cells, W. B. Saunders, Philadelphia.Google Scholar
  45. 45.
    Robertson J. D. (1899) On a new method of obtaining a block reaction in certain tissue elements of the central nervous system (Platinum method). Scott. Med. Surg. 4, 23–30.Google Scholar
  46. 46.
    Rome L. H., Bullock P. N., Chiappelli F., Cardwell M. C., Adinolfi A. M., and Swanson D. (1986) Synthesis of a myelin-like membrane by oligodendrocytes in culture. J. Neurosci. Res. 15, 49–65.PubMedCrossRefGoogle Scholar
  47. 47.
    Saneto R. P. and de Vellis J. (1985) Characterization of cultured rat oligodendrocytes proliferating in a serum-free chemically defined medium. Proc. Natl. Acad. Sci. 82, 3509–3513.PubMedCrossRefGoogle Scholar
  48. 48.
    Saneto R. P., Altman A., Knobler R. L., Johnson H. M., and de Vellis J. (1986) Interleukin 2 mediates the inhibition of oligodendrocyte progenitor cell proliferation in vitro. Proc. Natl. Acad. Sci. 83, 9221–9225.PubMedCrossRefGoogle Scholar
  49. 49.
    Skoff R. P. and Knapp P. E. (1991) Division of astroblasts and oligodendroblasts in postnatal rodent brain: Evidence for separate astrocyte and oligodendrocyte lineages. Glia 4, 165–174.PubMedCrossRefGoogle Scholar
  50. 50.
    Szuchet A., Polak P. E., and Yim S. H. (1986) Mature oligodendrocytes cultured in the absence of neurons recapitulate the ontogenic development of myelin membranes. Dev. Neurosci. 8, 208–221.PubMedCrossRefGoogle Scholar
  51. 51.
    Yim S. H., Szuchet S., and Polak P. E. (1986) Cultured oligodendrocytes: A role for cell-substratum interaction in phenotypic expression. J. Biol. Chem. 261, 11,808–11,815.PubMedGoogle Scholar

Copyright information

© The Humana Press Inc. Totowa, New Jersey 1992

Authors and Affiliations

  • Jean de Vellis
    • 1
  • Araceli Espinosa de los Monteros
    • 1
  1. 1.University of Southern California at Los AngelesLos Angeles

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