Vesicular Stomatitis Virus Pseudotypes of Retroviruses

  • Paul R. Clapham
Part of the Methods in Molecular Biology book series (MIMB, volume 8)


Pseudotype viruses are phenotypically mixed virions containing the genome or nucleocapsid of one enveloped virus and the surface or envelope (env) glycoproteins of another. This chapter will concentrate on vesicular stomatitis virus (VSV) pseudotypes retaining the VSV nucleocapsid but with alternative env glycoproteins derived from retroviruses.


Vesicular Stomatitis Virus Bovine Leukemia Virus Neutralization Epitope Pseudotype Virus Vesicular Stomatitis Virus Infectivity 
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  1. 1.
    Zavada, J., Cemy, L., Zavadova, Z., Bozonova, J., and Altstein, A D. (1979) A rapid neutralization test for antibodies to bovine leukemia virus with the use of rhabdovirus pseudotypes. J. Nati. Cancer Inst. 62, 95–101.Google Scholar
  2. 2.
    Weiss, R. A., Clapham, P., Nagy, K, and Hoshino, H. (1985) Envelope properties of human T-ell leukemia viruses. Curr. Top. Micokol. Immunol 115, 235–246.Google Scholar
  3. 3.
    Dalgleish, A. C, Beverly, P. C. L., Clapham, P. R, Crawford, D. H., Greaves, M. F., and Weiss, R. A. (1984) The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus. Nature 3, 763–767.CrossRefGoogle Scholar
  4. 4.
    Bruck, C, Portetelle, D., Burny, A., and Zavada, J. (1982) Topographical analysis by monoclonal antibodies of BLV-gp51 epitopes involved in viral functions. Virology 122, 353–362.PubMedCrossRefGoogle Scholar
  5. 5.
    Portetelle, D., Dandoy, C, Burny, A., Zavada, J., Siakkou, H., Gras-Masse, H., Drobecq, H., and Tartar, A. (1989) Synthetic peptides approach to identification of epitopes on bovine leukemia virus envelope glycoprotein gp51. Virology 169, 34–11.PubMedCrossRefGoogle Scholar
  6. 6.
    Choppin, P. W. and Compans, R. W. (1970) Phenotypic mixing of envelope proteins of the parainfluezna virus and vesicular stomatitisvirus. J. Virol 5, 609–616PubMedGoogle Scholar
  7. 7.
    Zavada. J. (1972) VSV pseudotype particles with thecoatofavian myeloblastosis virus. Nature New Biol. 240, 122.PubMedGoogle Scholar
  8. 8.
    Weiss. R. (1984) Experimental biology and assay of RNA tumor viruses, in KNATumor Viruses (Weiss, R., Teich, N., Varmus, H., and Coffin, J., eds.), CSH, New York, pp. 209–261.Google Scholar
  9. 9.
    Clapham, P., Nagy, K., and Weiss, R. A. (1984) Pseudotypes of human T-cell leukemia virus type 1 and neutralization by patients’ sera. Proc. Natl. Acad. Sci. USA 81, 2886–2889.PubMedCrossRefGoogle Scholar
  10. 10.
    Vaux, D. J. T., Helenius, A., and Mellman, I. (1988) Spike-nucleocapsid interaction in Semliki Forest virus reconstructed using network antibodies. Nature 336, 36–42.PubMedCrossRefGoogle Scholar
  11. 11.
    Zavada, J. (1982) The pseudotypic paradox. J. Gen. Virol. 63,15–54.PubMedCrossRefGoogle Scholar
  12. 12.
    Weiss, R. A. and Bennett, P. L. P. (1980) Assembly of membrane glycoproteins studied by phenotypic mixing between mutants of vesicular stomatitis virus and retroviruses. Virology 100, 252–274.PubMedCrossRefGoogle Scholar
  13. 13.
    Sommerfelt, M. A. and Weiss, R. (1990) Receptor interference groups among twenty retroviruses plating on human cells. Virology 176, 58–69.PubMedCrossRefGoogle Scholar
  14. 14.
    Clapham, P., Nagy, K, Cheingsong-Popov, R., Exley, M., and Weiss, R. A. (1983) Productive infection and cell free transmission of human T-cell leukemia virus in a nonlymphoid cell line. Science 222, 1125–1127.PubMedCrossRefGoogle Scholar
  15. 15.
    Maddon, P. J., Dalgleish, A. G., McDougal, J. S., Clapham, P. R., Weiss, R. A., and Axel, R. (1986) The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain. Cell 47, 333–348.PubMedCrossRefGoogle Scholar
  16. 16.
    Chesebro, B. and Wehrly, K. (1988) Development of a sensitive quantitative focal assay for human immunodeficiency virus infectivity. J. Virol. 6, 3779–3788.Google Scholar
  17. 17.
    Dalgleish, A. G., Chanh, T. C, Kennedy, R. C, Kanda, P., Clapham P. R., and Weiss, R. A. (1988) Neutralisation of diverse HIV-1 strains by monoclonal antibodies raised against a gp41 synthetic peptide. Virology 165, 209–215.PubMedCrossRefGoogle Scholar
  18. 18.
    McClure, M. C, Sommerfelt, M. A., and Weiss, R. A. (1990) The pH independence of mammalian retrovirus infection. J. Gen. Vtrol 71, 767–773.CrossRefGoogle Scholar

Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Paul R. Clapham
    • 1
  1. 1.Chester Beatty LaboratoriesInstitute of Cancer ResearchLondonUK

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