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Growth and Characterization of Poliovirus Antigen Chimeras

  • David J. Evans
  • Philip D. Minor
Part of the Methods in Molecular Biology book series (MIMB, volume 8)

Abstract

 Chapter 22 outlines the construction of engineered, full-length poliovirus cDNAs in which the region encoding a well-characterized antigenic site has been replaced by sequences of choice. This chapter briefly describes the methods used to generate, maintain, and characterize infectious chimeric viruses. These techniques include several developed during the course of poliovirus study that have recently been published (1). This overview describes modifications to these techniques where relevant, but concentrates on methods not detailed by Minor (1). The production and analysis of poliovirus antigen chimeras can be readily subdivided into three discrete areas.

Keywords

Antigenic Site Newborn Calf Serum Chimeric Virus Cesium Chloride Routine Growth 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • David J. Evans
    • 1
  • Philip D. Minor
    • 2
  1. 1.Department of MicrobiologyUniversity of ReadingReadingUK
  2. 2.National Institute for Biological Standards and ControlHertfordshireUK

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