Design and Construction of Poliovirus Epitope Expression Vectors

  • Karen L. Burke
  • Jeffrey W. Almond
  • David J. Evans
Part of the Methods in Molecular Biology book series (MIMB, volume 8)


We have developed the very safe and efficacious live-attenuated Sabin 1 poliovirus vaccine strain as a vehicle for the presentation of defined epitopes from foreign pathogens (1, 2, 3). Precise modification of the poliovirus capsid is made possible by the application of recombinant DNA technology to cloned cDNA copies of the viral genome, which are infectious for mammalian cells in culture (4).


Antigenic Site Chimeric Virus Unique Restriction Site Antigenic Domain Cassette Vector 
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Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Karen L. Burke
    • 1
  • Jeffrey W. Almond
    • 2
  • David J. Evans
    • 2
  1. 1.Institut für Mikrobiologie der Universitat UlmAbteilung VirologieUlmFederal Republic of Germany
  2. 2.Department of MicrobiologyUniversity of ReadingReadingUK

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