Assessment of Compounds for Anti-HIV Activity

  • Clive Patience
  • John Moore
  • Mark Boyd
Part of the Methods in Molecular Biology book series (MIMB, volume 8)


Since the identification of HIV (human immunodeficiency virus) as the retrovirus responsible for AIDS (acquired immunodeficiency syndrome), huge efforts have been made to identify compounds possessing antiviral activity. Many assay systems are available to measure various stages of the viral lifecycle. These are very convenient for assessing the antiviral activity of compounds. The assay systems often use T-cell lines expressing CD4 on their surface, since this molecule is an essential component of the receptor for the virus (1). The basic principle when testing for anti-HIV compounds involves the interaction of virus with test material under suitable culture conditions and the subsequent measurement of a parameter reflecting the amount of virus present and, thus, the degree of viral replication.


Antiviral Activity Antiviral Assay Blue Product Reaction Cocktail Template Primer 
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Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Clive Patience
    • 1
  • John Moore
    • 1
  • Mark Boyd
    • 1
  1. 1.Chester Beatty LaboratoriesInstitute of Cancer ResearchLondonUK

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