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Use of Escherichiu coli (E. coli) lacZ (β-Galactosidase) as a Reporter Gene

  • Grant R. MacGregor
  • Gamy P. Nolan
  • Steven Fiering
  • Mario Roederer
  • Leonard A. Herzenberg
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 7)

Abstract

Our understanding of the molecular mechanisms that govern gene expression has been facilitated by the ability to introduce recombinant DNA molecules into heterologous cellular systems both in vitro and in vivo. One approach to defining DNA sequences important in the regulation of gene expression is to place controlling elements (e.g., promoter/enhancer sequences) upstream of a DNA coding sequence, introduce these constructs into transgenic animals or cells in culture, and analyze the levels of gene product produced by the introduced construct. Ideally, such a reporter gene should encode a product that is stable, innocuous to the cell or organism in which it is being expressed, and should be readily detectable, even when present in small quantities.

Keywords

Histochemical Assay Accurate Quantitation Nylon Screen Staining Medium Single Viable Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© The Humana Press Inc., Clifton, NJ 1991

Authors and Affiliations

  • Grant R. MacGregor
    • 1
  • Gamy P. Nolan
    • 2
  • Steven Fiering
    • 2
  • Mario Roederer
    • 2
  • Leonard A. Herzenberg
    • 2
  1. 1.Institute for Molecular GeneticsHouston
  2. 2.Department of GeneticsStanford UniversityStanford

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