Abstract
Methods are described for performing a first analysis of newly prepared macromolecular crystals. Biochemical analysis using denaturing gel electrophoresis on dissolved crystals informs on the content of the crystal. Detailed protocols are given for mounting crystals in capillaries or fiber loops in preparation for analysis of their X-ray diffractive properties.
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References
Matthews, B. W. (1968) Solvent content of protein crystals. J. Mol. Biol. 33, 491–497.
Otwinowski, Z. and Minor, W. (1997) Processing of X-ray diffraction data collected in oscillation mode. In: Macromolecular Crystallography, 1.6 edit., Vol. 276, (Sweet, R. M. and Carter, C. W., eds.), Academic Press, New York, NY, pp. 307–326.
Pflugrath, J. W. (1999) The finer things in X-ray diffraction data collection. Acta Crystallogr. D. Biol. Crystallogr. 55, 1718–1725.
Leslie, A. G. W. (1992) Recent changes to the MOSFLM package for processing film and image plate data. Joint CCP4 + ESF-EAMCB Newsletter on Protein Crystallography 26.
Winn, M. D. (2003) An overview of the CCP4 project in protein crystallography: an example of a collaborative project. J. Synchrotron. Radiat. 10, 23–25.
Rossmann, M. G. and van Beek, C. G. (1999) Data processing. Acta Crystallographica D55, 1631–1653.
Sambrook, J. and Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Teng, T.-Y. (1990) Mounting of crystals for macromolecular crystallography in a free-standing thin film. J. Appl. Cryst. 23, 387–391.
Rodgers, D. (1997) Practical cryocrystallography. In: Macromolecular Crystallography, Vol. 276, (Carter, C. W. and Sweet, R., eds.), Academic Press, New York, NY, pp. 183–202.
Garman, E. F. and Schneider, T. R. (1997) Macromolecular crystallography. J. Appl. Cryst. 30, 211–237.
Hicks, L. M., Hunt, J. L., and Baxter, C. R. (1979) Liquid propane cold injury: a clinicopathologic and experimental study. The Journal of Trauma 19, 701–703.
Blundell, T. L. and Johnson, L. N. (1976) Protein Crystallography, Academic Press, New York, NY.
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Jeruzalmi, D. (2007). First Analysis of Macromolecular Crystals. In: Doublié, S. (eds) Macromolecular Crystallography Protocols. Methods in Molecular Biology™, vol 364. Humana Press. https://doi.org/10.1385/1-59745-266-1:43
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DOI: https://doi.org/10.1385/1-59745-266-1:43
Publisher Name: Humana Press
Print ISBN: 978-1-58829-902-4
Online ISBN: 978-1-59745-266-3
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