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First Analysis of Macromolecular Crystals

Biochemistry and X-Ray Diffraction

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Macromolecular Crystallography Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 364))

Abstract

Methods are described for performing a first analysis of newly prepared macromolecular crystals. Biochemical analysis using denaturing gel electrophoresis on dissolved crystals informs on the content of the crystal. Detailed protocols are given for mounting crystals in capillaries or fiber loops in preparation for analysis of their X-ray diffractive properties.

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References

  1. Matthews, B. W. (1968) Solvent content of protein crystals. J. Mol. Biol. 33, 491–497.

    Article  PubMed  CAS  Google Scholar 

  2. Otwinowski, Z. and Minor, W. (1997) Processing of X-ray diffraction data collected in oscillation mode. In: Macromolecular Crystallography, 1.6 edit., Vol. 276, (Sweet, R. M. and Carter, C. W., eds.), Academic Press, New York, NY, pp. 307–326.

    Google Scholar 

  3. Pflugrath, J. W. (1999) The finer things in X-ray diffraction data collection. Acta Crystallogr. D. Biol. Crystallogr. 55, 1718–1725.

    Article  PubMed  CAS  Google Scholar 

  4. Leslie, A. G. W. (1992) Recent changes to the MOSFLM package for processing film and image plate data. Joint CCP4 + ESF-EAMCB Newsletter on Protein Crystallography 26.

    Google Scholar 

  5. Winn, M. D. (2003) An overview of the CCP4 project in protein crystallography: an example of a collaborative project. J. Synchrotron. Radiat. 10, 23–25.

    Article  PubMed  CAS  Google Scholar 

  6. Rossmann, M. G. and van Beek, C. G. (1999) Data processing. Acta Crystallographica D55, 1631–1653.

    CAS  Google Scholar 

  7. Sambrook, J. and Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.

    Google Scholar 

  8. Teng, T.-Y. (1990) Mounting of crystals for macromolecular crystallography in a free-standing thin film. J. Appl. Cryst. 23, 387–391.

    Article  CAS  Google Scholar 

  9. Rodgers, D. (1997) Practical cryocrystallography. In: Macromolecular Crystallography, Vol. 276, (Carter, C. W. and Sweet, R., eds.), Academic Press, New York, NY, pp. 183–202.

    Chapter  Google Scholar 

  10. Garman, E. F. and Schneider, T. R. (1997) Macromolecular crystallography. J. Appl. Cryst. 30, 211–237.

    Article  Google Scholar 

  11. Hicks, L. M., Hunt, J. L., and Baxter, C. R. (1979) Liquid propane cold injury: a clinicopathologic and experimental study. The Journal of Trauma 19, 701–703.

    Article  PubMed  CAS  Google Scholar 

  12. Blundell, T. L. and Johnson, L. N. (1976) Protein Crystallography, Academic Press, New York, NY.

    Google Scholar 

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© 2007 Humana Press Inc.

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Jeruzalmi, D. (2007). First Analysis of Macromolecular Crystals. In: Doublié, S. (eds) Macromolecular Crystallography Protocols. Methods in Molecular Biology™, vol 364. Humana Press. https://doi.org/10.1385/1-59745-266-1:43

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  • DOI: https://doi.org/10.1385/1-59745-266-1:43

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-902-4

  • Online ISBN: 978-1-59745-266-3

  • eBook Packages: Springer Protocols

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