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Measuring Programmed Cell Death in Plants

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Apoptosis Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 282))

Abstract

Methods for the detection of programmed cell death (PCD) in plants are reviewed with references for different biochemical, microscopic, and molecular assays. A detailed description of three different methods for the detection of biotic or abiotic PCD in plant tissues is included. The reader is encouraged to use all three methods in parallel to obtain a reliable measure of PCD. Critical considerations are highlighted.

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Author information

Authors and Affiliations

  1. Department of Botany, Plant Sciences Institute, Iowa State University, Ames, IA

    Ludmila Rizhsky

  2. Virginia Bioinformatics Institute, Blacksburg, VA

    Vladimir Shulaev

  3. Department of Biochemistry, University of Nevada, Reno, NV

    Ron Mittler

Authors
  1. Ludmila Rizhsky
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  2. Vladimir Shulaev
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  3. Ron Mittler
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Editor information

Editors and Affiliations

  1. Institute of Child Health, University College London, London, UK

    Hugh J. M. Brady

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© 2004 Humana Press Inc.

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Rizhsky, L., Shulaev, V., Mittler, R. (2004). Measuring Programmed Cell Death in Plants. In: Brady, H.J.M. (eds) Apoptosis Methods and Protocols. Methods in Molecular Biology, vol 282. Humana Press. https://doi.org/10.1385/1-59259-812-9:179

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  • DOI: https://doi.org/10.1385/1-59259-812-9:179

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-873-8

  • Online ISBN: 978-1-59259-812-0

  • eBook Packages: Springer Protocols

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