Skip to main content
SpringerLink
Log in
Book cover

Heme, Chlorophyll, and Bilins pp 95–109Cite as

Analysis of Biosynthetic Intermediates, 5-Aminolevulinic Acid to Heme

  • Protocol

  • 5034 Accesses

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Chromatographic techniques are widely used for the analysis of heme and its precursors. Recent and continuing improvements in column packing materials for high-performance liquid chromatography (HPLC) have led to much better column efficiency and resolution. There have also been great advances in the direct coupling of liquid chromatography (LC), including capillary electrophoresis (CE), to mass spectrometry (MS) to provide highly sensitive and specific methods of analysis.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Bonnett, R., AA. Charalambides, K. Jones, I A. Magnus, and R.J. Ridge. 1978. The direct determination of porphyrin carboxylic acids. High-pressure liquid chromatography with solvent systems containing phase-transfer agents. Biochem. J. 173:693–695.

    PubMed  CAS  Google Scholar 

  2. Chiang, S.C.C. and S.F.Y. Li. 1997. Separation of porphyrins by capillary electrophoresis in fused-silica and ethylene vinyl acetate copolymer capillaries with visible absorbance detection. Biomed. Chromatogr. 11:366–370.

    Article  PubMed  CAS  Google Scholar 

  3. Li, F., C.K. Lim, and T.J. Peters. 1987. Separation and characterization of pentacarboxylic porphyrinogen isomers by high-performance liquid chromatography with electrochemical detection. Biochem. J. 243:421–423.

    Google Scholar 

  4. Li, F., C.K. Lim, and T.J. Peters. 1989. Preparation, high-performance liquid chromatographic separation and characterization of hexacarboxylic porphyrinogens. J. Chromatogr. 461:353–359.

    Article  PubMed  CAS  Google Scholar 

  5. Lim, C.K., F. Li, and T.J. Peters. 1986. High-performance liquid chromatography of uroporphyrinogen and coproporphyrinogen isomers with amperometric detection. Biochem. J. 234:629–633.

    PubMed  CAS  Google Scholar 

  6. Lim, C.K., F. Li, and T.J. Peters. 1987. High-performance liquid chromatography of type-III heptacarboxylic porphyrinogen isomers. Biochem. J. 247:229–232.

    PubMed  CAS  Google Scholar 

  7. Lim, C.K., F. Li, and T.J. Peters. 1988. High-performance liquid chromatography of porphyrins. A review. J. Chromatogr. Biomed. Appl. 429:123–153.

    Article  CAS  Google Scholar 

  8. Lim, C.K. and T.J. Peters. 1984. Urine and faecal porphyrin profiles by reversed-phase high-performance liquid chromatography in the porphyrias. Clin. Chim. Acta 139:55–63.

    Article  PubMed  CAS  Google Scholar 

  9. Lim, C.K., M.A. Razzaque, J. Luo, and P.B. Farmer. 2000. Isolation and characterization of protoporphyrin glycoconjugates from rat Harderian gland by HPLC, capillary electrophoresis and HPLC/electrospray ionization MS. Biochem. J. 347:757–761.

    Article  PubMed  CAS  Google Scholar 

  10. Lim, C.K., J.M. Rideout, and T.J. Peters. 1984. High-performance liquid chromatography of dicarboxylic porphyrins and metalloporphyrins: retention behaviour and biomedical applications. J. Chromatogr. 377:333–341.

    Article  Google Scholar 

  11. Lim, C.K., J.M. Rideout, and D.M. Samson. 1979. Determination of 5-aminolaevulinic acid and porphobilinogen by high-performance liquid chromatography. J. Chromatogr. 185:605–611.

    Article  PubMed  CAS  Google Scholar 

  12. Lord, G.A., J.L. Luo, and C.K. Lim. 1999. Capillary zone electrophoresis/mass spectrometry of 5-aminolaevulinic acid and porphobilinogen. Rapid. Comm. Mass Spec. 74:314–316.

    Google Scholar 

  13. Luo, J.L., J. Deka, and C.K. Lim. 1996. Determination of 5-aminolaevulinic acid dehydratase activity in erythrocytes and porphobilinogen in urine by micellar electrokinetic capillary chromatography. J. Chromatogr. 722:353–357.

    Article  CAS  Google Scholar 

  14. Luo, J. and C.K. Lim. 1993. Order of uroporphyrinogen III decarboxylation on incubation of porphobilinogen and uroporphyrinogen III with erythrocyte uroporphyrinogen decarboxylase. Biochem. J. 289:529–532.

    PubMed  CAS  Google Scholar 

  15. Luo, J. and C.K. Lim. 1995. Isolation and characterization of new porphyrin metabolites in human porphyria cutanea tarda and in rats treated with hexachlorobenzene by HPTLC, HPLC and liquid secondary ion mass spectrometry. Biomed. Chromatogr. 9:113–122.

    Article  PubMed  CAS  Google Scholar 

  16. Mauzerall, D. and S. Granick. 1956. The occurrence and determination of δ-aminolevulinic acid and porphobilinogen in urine. J. Biol. Chem. 219:435–446.

    PubMed  CAS  Google Scholar 

  17. Meyer, H.D., W. Vogt, and K. Jacob. 1984. Improved separation and detection of free porphyrins by high-performance liquid chromatography. J. Chromatogr. 290:207–213.

    Article  PubMed  CAS  Google Scholar 

  18. Rideout, J.M., D.J. Wright, and C.K. Lim. 1983. High performance liquid chromatography of uroporphyrin isomers. J. Liq. Chromatogr. 6:383–394.

    Article  CAS  Google Scholar 

  19. Rossi, E. and D.H. Curnow. 1986. Porphyrins, Ch. 10, p. 261–303. In C.K. Lim (Ed.), HPLC of Small Molecules. IRL Press, Oxford.

    Google Scholar 

  20. Weinberger, R., E. Sapp, and S. Moring. 1990. Capillary electrophoresis of urinary porphyrins with absorbance and fluorescence detection. J. Chromatogr. 516:217–285.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. MRC Bioanalytical Science Group, School of Biological and Chemical Sciences, Birkbeck College, University of London, London, England, UK

    Chang Kee Lim

Authors
  1. Chang Kee Lim
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Plant Sciences, University of Cambridge, Cambridge, UK

    Alison G. Smith

  2. Department of Biochemistry, University of Cambridge, Cambridge, UK

    Michael Witty

Rights and permissions

Reprints and permissions

Copyright information

© 2002 Humana Press, Totowa, NJ

About this protocol

Cite this protocol

Lim, C.K. (2002). Analysis of Biosynthetic Intermediates, 5-Aminolevulinic Acid to Heme. In: Smith, A.G., Witty, M. (eds) Heme, Chlorophyll, and Bilins. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-243-0:95

Download citation

  • DOI: https://doi.org/10.1385/1-59259-243-0:95

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-111-0

  • Online ISBN: 978-1-59259-243-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation