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Protocols for Neural Cell Culture pp 117–127Cite as

Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures

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Abstract

The study of glial cell development and function has been considerably enhanced by the development of methods to culture oligodendrocytes and astrocytes from central nervous system tissue. A primary mixed glial culture, composed mostly of astrocytes, oligodendrocytes, and microglia, is obtained when newborn disaggregated cerebral brain cells from rat are plated at high cell density (2 × 105/cm2) in serum-supplemented medium (McCarthy and de Vellis, 1980).

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Further Reading

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Author information

Authors and Affiliations

  1. Neuropsychiatric Institute, University of California at Los Angeles, Los Angeles, CA

    Ruth Cole & Jean de Vellis

Authors
  1. Ruth Cole
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  2. Jean de Vellis
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Editor information

Editors and Affiliations

  1. Department of Anatomy and Cell Biology, School of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada

    Sergey Fedoroff  & Arleen Richardson  & 

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© 2001 Humana Press Inc., Totowa, NJ

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Cole, R., de Vellis, J. (2001). Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures. In: Fedoroff, S., Richardson, A. (eds) Protocols for Neural Cell Culture. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-207-4:117

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  • DOI: https://doi.org/10.1385/1-59259-207-4:117

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-902-5

  • Online ISBN: 978-1-59259-207-4

  • eBook Packages: Springer Book Archive

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