Abstract
Antisera are frequently used tools for the characterization of proteins and peptides because they provide unique information about the structural features of antigens. Proteins that share structural similarities can be identified by serological cross-reactions. The Western blotting technique combines two steps that are characteristic for immunoaffinity chromatography (1). One is the preparation of monospecific antibodies against a particular antigen and the second is the testing of their reactivity with the same or different antigens from any source of antigenic material.
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References
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© 2002 Humana Press Inc., Totowa, NJ
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Hammerl, P., Hartl, A., Freund, J., Thalhamer, J. (2002). Detection of Serological Cross-Reactions by Western Cross-Blotting. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1385/1-59259-169-8:1025
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DOI: https://doi.org/10.1385/1-59259-169-8:1025
Publisher Name: Humana Press
Print ISBN: 978-0-89603-940-7
Online ISBN: 978-1-59259-169-5
eBook Packages: Springer Book Archive