Abstract
Screening for mutations prior to sequencing can reduce the time and costs of identifying mutations. When the DNA sequence is known, the technique of detecting mutations as single-stranded conformational polymorphisms (SSCP) is a convenient method of screening for possible mutations. SSCP was originally developed by Orita et al. (1). It has the ability of detecting a single base change, and has been applied to a number of genes, including the insulin receptor (2), GLUT 4 (3), glucokinase (4), and the mitochondrial genome (5).
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© 2000 Humana Press Inc., Totowa, NJ
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Saker, P.J. (2000). Mutation Screening Using PCR-SSCP. In: Rapley, R. (eds) The Nucleic Acid Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-038-1:695
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DOI: https://doi.org/10.1385/1-59259-038-1:695
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-459-4
Online ISBN: 978-1-59259-038-4
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