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In Vitro Toxicity Testing Protocols pp 219–226Cite as

Cell Culture Phototoxicity Test

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 43))

Abstract

Human A431 cells and mouse 3T3 cells are exposed in culture to UV light both in the presence and absence of test compound. Phototoxicity is expressed as a decrease in cell viability as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay.

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References

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Author information

Authors and Affiliations

  1. Zeneca Pharmaceuticals, Macclesfield Cheshire, UK

    Paul A. Duffy

Authors
  1. Paul A. Duffy
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Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    Sheila O’Hare  & Chris K. Atterwill  & 

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© 1995 Humana Press Inc , Totowa, NJ

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Duffy, P.A. (1995). Cell Culture Phototoxicity Test. In: O’Hare, S., Atterwill, C.K. (eds) In Vitro Toxicity Testing Protocols. Methods in Molecular Biology™, vol 43. Humana Press. https://doi.org/10.1385/0-89603-282-5:219

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  • DOI: https://doi.org/10.1385/0-89603-282-5:219

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-282-8

  • Online ISBN: 978-1-59259-530-3

  • eBook Packages: Springer Protocols

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