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Organotypic Slice Explant Roller-Tube Cultures

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Practical Cell Culture Techniques

Part of the book series: Neuromethods ((NM,volume 23))

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Abstract

Within the past decade, organ cultures have reemerged as an important complement to in vivo studies for examining regulatory mechanisms in various neuronal systems. The principle behind organ cultures is to maintain a tissue explant in a state as close as possible to that found in vivo, i.e., to preserve some aspect of the spatial, structural, and/or synaptic organization of the original tissue. As a result, such cultures are often referred to as organotypic and are valuable tools to study organized, yet relatively isolated neuronal systems.

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Author information

Authors and Affiliations

  1. Laboratory of Neurochemistry, NINDS, NIH, Bethesda, MD

    Susan Wray

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  1. Susan Wray
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Editor information

Editors and Affiliations

  1. University of Saskatchewan, Saskatoon, Canada

    Alan A. Boulton

  2. University of Alberta, Edmonton, Canada

    Glen B. Baker

  3. University of Saskatchewan, Saskatoon, Canada

    Wolfgang Walz

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© 1992 The Humana Press Inc. Totowa, New Jersey

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Wray, S. (1992). Organotypic Slice Explant Roller-Tube Cultures. In: Boulton, A.A., Baker, G.B., Walz, W. (eds) Practical Cell Culture Techniques. Neuromethods, vol 23. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-214-0:201

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  • DOI: https://doi.org/10.1385/0-89603-214-0:201

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-214-9

  • Online ISBN: 978-1-59259-628-7

  • eBook Packages: Springer Protocols

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