Abstract
On account of their relatively small, defined genomes, viruses have long been a convenient model system in which to study eucaryotic gene expression. Usually, viral infection is followed by early gene expression, which allows the subsequent processes of viral DNA replication and late gene expression to proceed. All these processes must be precisely regulated and involve both virally encoded and host proteins.
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References
Berk, A. J. (1986) Adenovirus promoters and E1A activation. Annu Rev. Genet 20, 45–79.
Ferguson, D., Kippl, D., Andrisani, O., Jones, N., Westphal, H., and Rosenberg, M (1985) E1A 13S and 12S mRNA products made in E. Coh both function as nucleus-localized transcription activators but do not directly bind DNA. Mol. Cell Biol. 5, 2653–2661.
Yoshinaga, S., Boulanger, P.A., and Berk, A.J. (1987) Resolution of human transcription factor TF IIIC into two functional components. Proc Natl. Acad. Sci. USA 84, 3585–3589.
Hoeffler, W. K, Kovelman, R., and Roeder, R. G. (1988) Activation of transcription factor IIIC by the adenovirus E1A protein. Cell 53, 907–920.
Kovesdi, I., Reichel, R., and Nevins, J. R. (1986) Identification of a cellular transcription factor involved in E1A transactivation. Cell 45, 219–228.
Raychandhuri, P., Rooney, R., and Nevins, J. R., (1987) Identification of an E1A-ihducible cellular factor that interacts with regulatory sequences within the adenovirus E4 promoter. EMBO J. 6, 4073–4081.
Lee, K. A. W., Hai, T-Y., SivaRamon, L., Thimmappaya, D., Hurst, H. C, Jones, N. C, and Green, M. R. (1989) A cellular transcription factor ATF activates transcription of multiple E1A-inducible adenovirus early promoters. Proc. Natl. Acad. Sci. USA 84, 8355–8359.
Simon, M. C., Fison, T. M., Benecke, B. J., Nevins, J. R., and Heintz, N. (1988) Definition of multiple, functionally distinct TATA elements, one of which is a target in the HSP 70 promoter for E1A regulation. Cell 52, 723–729.
Lee, K. A. W. and Green, M. R., (1987) A cellular transcription factor E4F1 interacts with an E1A-inducible enhancer and mediates constitutive enhancer function in vitro. EMBO J. 6, 1345–1353.
Dignam, J. D., Lebowitz, R. M., and Roeder, R. G. (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Aads Res. 11, 1475–1489.
Maxam, A. M. and Gilbert, W. (1980) Sequencing end-labelled DNA with base specific chemical cleavages. Methods Enzymol. 65, 499–560
Kadonaga, J. T. and Tjian, R. (1986) Affinity purification of sequence-specific DNA binding proteins. Proc. Natl. Acad. Sci. USA 83, 5889–5893.
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© 1991 The Humana Press Inc., Clifton, NJ
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Hurst, H.C. (1991). Analysis of Protein Factors that Interact with Adenovirus Early Promoters. In: Collins, M.K.L. (eds) Practical Molecular Virology. Methods in Molecular Biology, vol 8. Humana Press. https://doi.org/10.1385/0-89603-191-8:141
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DOI: https://doi.org/10.1385/0-89603-191-8:141
Publisher Name: Humana Press
Print ISBN: 978-0-89603-191-3
Online ISBN: 978-1-59259-495-5
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